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Click here or scroll down to respond to this candidateSummary- Scientist with Knowledge and technical expertise in molecular and cell biology,genetics, immunology, biochemistry, clinical research, in neurodegenerative diseases.- Expertise in cell culture, microscopy, tissue/cell processing; in-vivo and in-vitro projects.- Expertise in planning, procurement and project management.- Extensive experience in multicultural environment with analytical skills in cuttings edge technologies and the ability to solve problems to meet the timeline planned in a fast-paced work environment.- Proficiency in maintain records and any type of the documentation.- Ability to work independently as well as team member.- Expertise to lead projects and delegate tasks, strategic planning adaptability, empathy and good communication.- Experience in cGMP, GLP and GCP guidelines.Work ExperienceScientist II Enzo Life Sciences Biotech Company, July 2022 Current.- Manufacture, production, validation and quality control of more than 90 products (Bulks, Finish goods, kits) that they were sold in America, Europe and Asia.- The products are drug discovery kits, apoptosis kits, Proteostat Kits, Standars, monoclonal and polyclonal antibodies, protein purification and bioprocessing; enzymes development; RNA/DNA probes.- Expertise in several laboratory techniques as SDS Page, western blot, Protein purification (FPLC-AKTA system), flow cytometry, Elisa, enzyme activity assay, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), multiplex IHC, In-situ hybridization (ISH, FISH), molecular pathology, Endotoxin detection, cell proliferation assay, NGS, PCR, and qPCR.- Research and development experience.- Expertise In vitro workflow using CHOK1, HeLa, 293Hek and Jurkat cells. mammalian cell culture and Nuclear HeLa cell extraction. Rolando Xavier Avils-Reyes, PhDPHONE NUMBER AVAILABLEEMAIL AVAILABLE- Data analysis, write SOPs, batch records and presentation of results. Scientist I University of Wisconsin Madison, USA (March 2020-July 2022).- Design, run and analyze experiments related with Alzheimer disease, working with transgenic mice, in vitro cells and AAVs.- Expertise in several techniques and protocols as isolation of DNA/RNA, RNAseq, RT-PCR, PCR and qPCR, western blot, Immunohistochemistry (IHC), immunofluorescence (IF).- Acquisition, management, sharing and interpretation of pathology information, including slides and data.- Transfection of the AAV into neuronal and astrocytes cell culture, hiPSC Cell system. AAV retro-orbital injection in mice, harvest cortex, hippocampus, cerebellum, spinal cord; process the tissue for protein, DNA/RNA, IHC, IFC, enzyme analysis.- Data analysis; presentation of results. Write manuscripts and grants. Research fellow, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA, March 2020 March 2021.- Design and run experiments in vivo and in vitro models (hiPSC Cell system) in an experimental model of neurogenesis in trauma brain injury.- Techniques: isolation of DNA/RNA, PCR, RT-PCR, PCR, and qPCR, western blot, IHC, IFC. Write manuscripts.- Data analysis, results presentation.Research Associate, School of Pharmacy, University of Wisconsin Madison, USA, (August 2016 March 2020).- Design, run and analyze experiments in vivo and in vitro models. Techniques used were isolation of DNA/RNA, RNAseq, PCR, RT-PCR, PCR, and qPCR, western blot, Immunohistochemistry (IHC), immunofluorescence (IFC). Acquisition, management, sharing and interpretation of pathology information, including slides and data.- Expertise in vitro experiments doing transfection of the AAV into neuronal and astrocytes cell culture. Retro-orbital AAV-injection in mice, harvest tissue: cortex, hippocampus, cerebellum, spinal cord; process the tissue for protein, DNA/RNA, IHC, IFC, and enzyme activity. Assay development, Write projects, manuscripts. Data analysis, results presentation.Scientist at BioAdvanced Cia. Ltda Quito, Ecuador (Jan 2013 - June 2016).- Scientist: R&D and quality control of endotoxins. hiPSC Cell system, FPLC. Vital Clinical laboratory, Quito, Ecuador (Jan 2013 - June 2016):- Clinical Laboratory Manager expertise in test of Molecular Biology, Clinical Biochemistry, biological liquids, urinalysis & parasitology, immunohematology, cytology, genetics, clinical bacteriology. National Scientific Council, Quito, Ecuador, (Jan 2012 June 2014).- Research Project Management of Genetic project of Huntington Disease in mice and in human samples who has the pathology. Expertise in techniques DNA/RNA, PCR, RT-PCR, IHC, IFC. Write projects, manuscripts. Data analysis, results presentation.Education- Professional certification 21 CFR Part 820 - Medical Device cGMP, Biopharma Institute- Postdoctoral research fellow, Johns Hopkins University, Baltimore, Maryland, USA.-Postdoctoral Research Associate, School of Pharmacy, University of Wisconsin Madison, USA.-PhD, School of Medicine, University of Buenos Aires Argentina.-Bachelor of Science, School of Bioanalysis of Pontifical Catholic University in Quito, Ecuador. Techniques, Software & Instrumentation- Assay development. - FPLC- Mammalian cell culture - RT-PCR, PCR- Adeno-associated virus (AAV) - dPCR, qPCR- hiPSC - RNA/DNA isolation- CHO, Hek, Jurkat, HeLa, HEK cells - Denovix cell drop.- In vivo based assay - Elisa- Enzyme Activity assay - genotyping- Immunohistochemistry - Optical microscopy- Immunofluorescence - Scope to dissect animals- In situ Hybridization ISH, FISH - Confocal microscopy- Western blot (tris glycine; tricine) - Cryopreservation- Handling of mice colony - Inverted microscope- Use of different transgenic mice - Breeding mice- Patients samples (clinical research) - GraphPad- image J - Adobe Acrobat- Panther Gene list analysis - FunRich- Data analysis - Microsoft office, Dynamics (Nvision)- RNA-seq data analysis - Mac or Microsoft- Protein analysis (Densitometry) - Plasmids- GLP, GMP, GCP - NGS- Single cell sequencing - Labeled DigX and Biotin- Drug Discovery Kit - Flow Cytometry- SOPS; Batch recordsProfessional Achievements-Manufacturing, production, quality control of more that 80 different biomolecular products to research use only in a Biotech company-Completed the bachelor, PhD and Postdoctoral projects as a planner, procurement, and execution.-Published several peer-review articles in scientific journals on topics related to neurobiology.-Assistance at thirty scientific meetings where I have participated as first author or co-author in posters.-Presentation of twenty-one lectures of results obtained in different projects that I have participated.-Nine Grants participation as Ph.D. student, postdoctoral research associate and principal investigator.-Fourteen awards-Reviewer of four scientific journals-Innovation of methodologies at the bench of two technical protocols-Team worker and collaborations with laboratories and institutes partners.-Experienced in different lab equipment and supplies.-Initiatives in new research projects and mentoring undergraduates, graduate, and PhD students in the laboratory.-Mentor of school studentsAffiliations & Hobbies-Alzheimer Association AAIC, 2018- at present-International Society for Neurochemistry, (ISN) 2011 at present.-American Society for Neurochemistry, (ASN) 2009 at present-Argentina Society for Neuroscience, (SAN) 2007 at present-Society for Neuroscience (SfN), 2008, at present-International Brain Organization (IBRO) 2007- at present-Hobbies: mountain biker, gym exercises and traveler. Humanitarian work-Medrite Urgen Care, New York, 2023, Humanitarian relieve work.-International Mentoring, Madison, WI. (Mentor who interact with school, community, and families).Linkshttps://intentionalmentoringmadison.orghttps://LINKEDIN LINK AVAILABLE |
