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CURRICULUM VITAENAME: Chowdhury, SayanEMAIL ID: EMAIL AVAILABLE, EMAIL AVAILABLE CONTACT: PHONE NUMBER AVAILABLE POSITION TITLE: Research AssociateSponsorship needed: NOEDUCATION/ TRAINING:INSTITUTION ANDLOCATIONDEGREE(if applicable)START DATEMM/YYYYEND DATEMM/YYYYFIELD OF STUDYJohns Hopkins School ofmedicine, Baltimore, MD,USAResearchAssociateFaculty11/2021 Current Clinical HematologicmalignancyJohns Hopkins School ofmedicine, Baltimore, MD,USAPost-DoctoralFellow10/2014 08/2021 Molecular BiologyUniversity of Calcutta(IICB), Kolkata, WestBengal, India.Ph.D. 08/2008 08/2014 Molecular ParasitologyUniversity of Calcutta,Kolkata, West Bengal,India.M.Sc. 08/2006 05/2008 Biophysics, MolecularBiology and GeneticsUniversity of Calcutta,Kolkata, West Bengal,India.B.Sc. 08/2003 05/2006 Microbiology (Major),Chemistry & Zoology(General)A. Personal StatementI am a Research scientist at the Johns Hopkins School of Medicine, with more than three years of experience in immuno-oncology, biomarker identification in hematologic malignancies and six years of post-doctoral experience in molecular biology of disease pathogenesis. My long-term research goals involve understanding the complex role of immunological battle in a setting of host-disease relationship. I am building on my knowledge of immunology and technical expertise by focusing on understanding interplay of co-signaling molecules in Acute leukemia especially in the context of various immunotherapy and Adoptive Cell Therapy with allogeneic Marrow Infiltrating Lymphocytes in several different clinical trials. My area of research encompasses studying the molecular signature on the T cells and myeloid leukemia cells, and in understanding the immunometabolic role of myeloid and lymphoblastic leukemia in evading T cell mediated immune response. In these circumstances, I learned to plan, develop, organize and execute a project from scientific cornerstone as well as human resources. I perform well working alone, but I strongly believe in collaborating with others as a teamwork for successful completion of a project. During my first postdoctoral training, my primary focus was to understand interplay of inositol phosphate signaling in the context of neoplastic transformation/solid tumor model, immunolgical signaling pathway in macrophages as well as host pathogen relationship in human cytomegalovirus infection. As the part of a team, I also deciphered the role of a key enzyme of the inositol pathway in transcriptional regulation of autophagy and mTOR pathway.During my PhD, my area of study was in host-pathogen relationship in the protozoan parasite, Leishmania infection and developing potential drug candidates, for which I received several academic awards. I also gathered experience to mentor other graduate and undergraduate students during my research career. I always apply positive attitude, being target driven, open to challenges, and love to network. B. Technical Skills:Cell Culture: Co-culturing T cell with tumor cells in presence or absence of transwell to study contact dependent or independent phenotypic changes in T cellsMaintenance, storage and propagation of various bacterial, yeast, leishmanial, insect, mammalian primary(macrophage, lymphocytes, neuronal, colon) and cancer cell lines (adherent and non-adherent). Lenti, retro, adeno, cytomegalo -virus titer preparation and infection in cell lines. Transfection of shRNA, siRNA and cDNA construct in mammalian cells (primary and cell lines). Transfection and expression of tagged proteins in bacterial and mammalian cells.Immunology: Isolation of PBMC and BMMC from whole human blood and bone marrow (including clinical samples) followed by proper cryopreservation with high cellular viability Designing, staining and processing 16-color panel for multiparametric flow cytometry. Running flow cytometry on conventional cytometer like BD Fortessa or LSRII. Magnetic activated cell sorting. Preparing samples for sorting on BD FACS AriaII or Mo Flow. Preliminary data analysis on Flow Jo, FACS Diva, GraphPad Prizm, followed by complex bioinformatics analysis on Cytobank. Working with marrow infiltrating lymphocytes and their in-vitro functional assays. Designing in vitro co-culture experiments with T cells from Leukemia patients and Myeloid leukemia cells, under autologous or allogenic setting. Detection of the cytokine level in cell supernatant either in vitro or ex vivo post stimulation by sandwich ELISA using standard kit. Detection of IgG level in serum by ELISA to understand the humoral response. Flow-based Intracellular Interleukin assay post PMA Ionomycin treatment and golgi block Brefeldin A. Apoptosis assay, Cell cycle and division assay by Flow cytometry Mitochondrial functional Assays (Membrane potential with TMRE, Superoxide by MtSOX dye, Mass by Mitotracker) Assessment of the Energy Metabolism of sorted CD4, CD8 and blast/monocyte cells from diagnosed and remission/relapsed patients of Leukemia using SeaHorse.Cell Biology Techniques: Fluorescence techniques using fluorescence microscope and confocal microscope. Preparation of samples for electron microscopy, fluorescence and confocal microscopy. Cell proliferation assay or cell killing assay. Telomerase Assay, Cell migration Assay, Soft Agar colony formation assay, tumoroid assay, Senescence related experiments and markers expression and tumor/cancer related studies.Molecular Biology: Cloning of genes in different mammalian cloning vectors and purification of protein from leishmania cells and mammalian cells by Hydroxiapatite and Phosphocellulose column, Ni2+-NTA chromatography Isolation of kit based, or Phenol-Chloroform-Ethanol based RNA from cultured cells or tissues, Quantitative Polymerase Chain Reaction. Blottings (Southern, Northern and Western, EMSA) and Immunoprecipitation (IP) and Chromatin- Immunoprecipation (ChIP) pull-down assay, Co-immunoprecipitation in ectopic system in mammalian cells Probe labelling for southern/northern blot by T4 Polynucleotidase kinase (T4PNK) reaction. DNA relaxation assays with topoisomerase I and plasmid DNA. Subcellular fractionation and Purification of mitochondria, Nucleus from mammalian cells or Leishmania cells by differential centrifugation or ultracentrifugation. Polymerase chain reaction and RT- PCR, Real time PCR. Sequencing of DNA by automated DNA sequencer from Applied Biosystem.Animal Model: Xenograft tumor model in SCID and Nude mice. In vivo murine cytomegalovirus infection model in pups. Leishmania infection via intracardial and tail-vein in mice and hamster Isolation of peritoneal and bone marrow macrophages from Balb/c, C57B/6 mice and lymphocyte preparation Liver and Spleen isolation, splenocyte preparation, mRNA preparation from macerated liver and spleen. C. Positions and HonorsPositions and Employment2014  2020 Postdoctoral Research Scholar, Solomon H Snyder Laboratory, Johns Hopkins Medical Institute, Department of Neuroscience, Baltimore, Maryland 2021-2024 Research Faculty/Associate, Department of Hematology Oncology, Johns Hopkins Medical Institute, Department of Neuroscience, Baltimore, Maryland Honors2007 Qualified for National Eligibility Test (NET) for Junior Research Fellowship for PhD, Council for Scientific and Industrial Research (Govt. of India) 2008 Qualified for National Eligibility Test (NET) for Junior Research Fellowship for PhD, Council for Scientific and Industrial Research (Govt. of India) 2008 Qualified in Graduate Aptitude Test in Engineering (GATE) for M. Tech, HRD & Indian Institute of Technology (Govt. of India)2011-2013 Qualified for Senior Research Fellowship (SRF) for PhD, Council for Scientific and Industrial Research (CSIR, Govt. of India)D. Research Experience1. Complex cell culture methods involving co-culture of T cells and tumor cells: a. FACS-sorted BM-T cells and AML blasts from de novo patients were co-cultured to study effects of blasts on the fitness of T cells. Blast-mediated T cell dysfunctionality was examined, and biomarkers were identified using multicolored flow cytometry.b. T cell and blast co-culture was done in either in direct contact or separated by trans well inserts, to test the modifications in T cell immunophenotype between direct contact with blasts or soluble factors from the blasts.c. Studied anti-CD3/CD19 BiTE (Blinatumomab) mediated, and T cell redirected killing of cultured cells(NUM6), to compare the anti-tumor efficacy between senescent and activated T cells from ALL patients. 2. Immunophenotyping of T cells and tumor cells from AML and ALL patients a. Whole blood from paired patient samples from de novo to different treatment timepoints, until the end of study was used to isolate the BMMC and PBMC and stored in batches. b. T cells from the batch were immunophenotyped for the following biomarkers:- T cell dysfunctionality: exhaustion and senescence- T cell activation- Transcription factors- Cytotoxicityc. Blast cells from the patients at de novo or at relapse pre- or post-transplant were also immunophenotyped for the corresponding ligands.d. Patients with different prognostic outcomes were compared for the evaluated biomarkers and the various predictive and prognostic biomarkers were identified in the process. Post-doctoral projects:1. Role of Inositol phosphate multikinase (IPMK) in neoplastic transformation of fibroblast. 2. Role of IPMK in human cytomegalovirus (HCMV) infection. 3. Elucidating the relation between IPMK and adult neurogenesis and memory in mice through ciliogenesis pathway.4. Role of IPMK in macrophage biology and lymphocyte development. Ph.D. projects:1. Novel betulin derivatives (triterpenoid) as antileishmanial agents with mode of action targeting Type IB DNA topoisomerase.2. Triterpenoid triggers Metacaspase dependent Endonuclease G mediated cell death in Unicellular Protozoan Parasite Leishmania donovani.3. Development of derivatives of 3, 3'-Diindolylmethane as potent Leishmania donovani bi-subunit topoisomerase IB poisons.4. Niranthin, a Lignan isolated from Phyllanthus amarus, abrogates Leishmania donovani replication by poisoning topoisomerase IB of parasite and ameliorates Th1 response to cure infected-BALB/c mice. 5. Molecular events leading to adaptive chemoresistance in progressively adapted flavone-resistant Leishmania.D. PublicationsList of publications:1. Mazziotta F, Biavati L, Rimando J, Rutella S, Borcherding N, Parbhoo S, Mukhopadhyay R, Knaus HA, Chowdhury S, Valent P, Hackl H, Borrello IM, Blazar BM, Hatzi K, Gojo I and Luznik L (2024) CD8+ T-cell Differentiation 1 and Dysfunction Inform Treatment Response in Acute Myeloid Leukemia. Blood. 2024 May 22:bloodPHONE NUMBER AVAILABLE2. Rimando J, Biavati L, Mazziota F, Chowdhury S, Webster J, Ali A, Dezern A, Levis M, Zahurak M, Ambinder R, Huff CA, Gojo I, Jones RJ, Borrello I and Luznik L (2024) A phase I/II trial of activated donor- derived marrow-infiltrated-lymphocytes to treat relapsed hematologic malignancies after allogeneic hematopoietic cell transplantation with post-transplantation cyclophosphamide as graft-versus-host disease prophylaxis. (Manuscript under revision)3. Guha P, Tyagi R, Chowdhury S, Reilly L, Fu C, Xu R, Resnick AC and Snyder SH (2019) IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. Cell Rep. 26(10):2692-2703.e7.4. Chowdhury S, Guha P, Tyagi R and Snyder SH (2024). IPMK is the master regulator of neoplastic transformation in primary fibroblasts. (manuscript under preparation). 5. Chowdhury S and Snyder SH (2024). Role of IPMK during cytomegalovirus entry. (manuscript under preparation)6. Chowdhury S, Mukherjee T, Mukhopadhyay R, Mukherjee B, Sengupta S, Chattopadhyay S, Jaisankar P, Roy S and Majumder HK (2012) Niranthin, a Lignan, intercepts Leishmania donovani replication by poisoning topoisomerase IB of parasite and ameliorates Th1 response to cure infected-BALB/c mice. EMBO Mol Med, 4:1126-43.7. Roy A#, Chowdhury S#, Sengupta S, Mandal M, Jaisankar P, D'Annessa I, Desideri A and Majumder HK(2011) Development of derivatives of 3, 3'-diindolylmethane as potent Leishmania donovani bi-subunit topoisomerase IB poisons. PLoS One, 6(2011):e28493. (#equal contribution). 8. Chowdhury S, Mukherjee T, Sengupta S, Chowdhury SR, Mukhopadhyay S and Majumder HK (2011) Novel betulin derivatives as antileishmanial agents with mode of action targeting type IB DNA topoisomerase. Mol Pharmacol, 80(2011):694-703.9. Chowdhury S, Mukherjee T, Chowdhury SR, Sengupta S, Jaisankar P, Mukhopadhyay S and Majumder HK (2014) Disuccinyl betulin triggers Metacaspase Dependent Endonuclease G Mediated Cell Death in Unicellular Antimicrob Agents Chemother, 58(4):2186-201. 10. Chowdhury S, Mukhopadhyay R, Saha S, Mishra A, Sengupta S, Roy S and Majumder HK (2014) Flavone resistant Leishmania donovani over expresses MRP2 transporter in the parasite as well as on the host macrophages to circumvent the flavone-mediated cell death. J Biol Chem, 289:16129-47. 11. Chowdhury S, Mukhopadhyay R, Mukherjee B, Mukherjee S, Naskar K, Sundar S, Dujardin JC, Majumdar HK and Roy S (2021) Sodium stibogluconate in combination with flavone quercetin reverses the resistance phenotype in clinical antimony resistant Leishmania donovani: implication in therapy. (manuscript under preparation).12. Mukhopadhyay B, Mukhopadhyay R, Bannerjee B, Chowdhury S, Mukherjee S, Naskar K, Allam US, Chakravorty D, Sundar S, Dujardin JC and Roy S (2013) Antimony resistant Leishmania donovani upregulates multi drug resistant protein1 in an IL-10 dependent manner by exploiting the TLR2 innate receptor. Proc Natl Acad Sci USA, 110(2013):E575-82.13. Saha S, Mukherjee T, Chowdhury S, Mishra A, Chowdhury SR, Jaisankar P, Mukhopadhyay S and Majumder HK (2013) The lignan glycosides lyoniside & saracoside poison the unusual type IB topoisomerase of Leishmania donovani and kill the parasite both in vitro and in vivo. Biochem Pharmacol, 2013 Oct 14. doi:pii: S0006-2952PHONE NUMBER AVAILABLE/j.bcp.2013.10.004.14. Sharma G, Chowdhury S, Sinha S, Majumder HK and Kumar SV (2013) Anti leishmanial evaluation of bis- lawsone analogs and DNA Topoisomerase-I inhibition studies. J Enzym Inhib Med Ch, 29:185-9. 15. Mukherjee T, Chowdhury S, Kumar A, Majumder HK, Jaisankar P and Mukhopadhyay S (2012) Saracoside: A New Lignan Glycoside from Saraca indica, a Potential Inhibitor of DNA Topoisomerase IB. Nat Prod Commun, 7:767-769.16. Sengupta S, Chowdhury S, Bosedasgupta S, Wright CW and Majumder HK (2011) Crytolepine-Induced Cell Death of Leishmania donovani Promastigotes Is Augmented by Inhibition of Autophagy. Mol Biol Int, 2011:187850.17. Mishra A, Vinayagam J, Saha S, Chowdhury S, Roychowdhury S, Jaisankar P and Majumder HK (2014) Isobenzofuranone derivatives exhibit antileishmanial effect by inhibiting type II DNA topoisomerase and inducing host response. Pharmacol Res Perspect, 2(6):e00070. 18. Paul J, Naskar K, Chowdhury S, Chakraborti T and De T (2014) TLR mediated GSK3 activation suppresses CREB mediated IL-10 production to induce a protective immune response against murine visceral leishmaniasis. Biochimie, 107 Pt B:235-46.Conference attended:Poster presentation:1. Different Subsets of Exhausted and Senescent-like T Cells Correlate with Response and Survival in Patients with Acute Myeloid Leukemia (AML) Undergoing Chemotherapy (2022). Mazziotta F, Biavati L, Parbhoo S, Chowdhury S, Rimando J, Borrello IM, Rutella S, Hatzi K, Blazar BM, Gojo I, Luznik L. (64th ASH Annual Meeting Abstracts) [Blood (2022) 140 (Supplement 1): 91329133] 2. Anti-inflammatory action of heme oxygenase mediated by binding and down-regulating nitric oxide synthase at the 46th Annual Meeting of Society for Neuroscience, held at San Diego, CA on November 12-16, 2016.3. "Derivatives of Pentacyclic Triterpenoid, Betulin, as Antileish- manial Agents Targeting DNA Topoisomerase IB of Leishmania" at International Symposium on Modern Biology in 21st Century: Teaching and Research held at University of Calcutta, Kolkata, India held on December 7th and 8th, 2010. 4. "Detail Study of the Mechanism of Inhibition of Unusual Bi- Subunit Topoisomerase IB from Leishmania donovani by Some Newly Synthesized Betulin Derived Chemicals: A selective Approach to Cure Leishmaniasis" at the 18th West Bengal State Science and Technology Congress held at Narendrapur, Kolkata, India held on 28th February to 1st March, 2011. 5. "Niranthin, a Lignan from Phyllanthus amarus Poisons Leish- mania donovani Type IB Topoisomerase: A Potential Candidate as Antileishmanial Agent" at the XXXV All India Cell Biology Conference (AICB) & Symposium on the Membrane Dynamics and Disease held at Bhubaneswar, Orissa, India on 16th to 18th December, 2011.6. "Laboratory-grown flavone resistant Leishmania over expresses MRP2 transporter on parasite as well as host in both stages of their life-cycle to circumvent the flavone-mediated cell death" at the 81st Annual Meeting of The Society of Biological Chemists (India) and Symposium on Chemistry and Biology: Two Weapons Against Disease held at Science City Auditorium Complex, Kolkata, India on November 8-11, 2012. 7. "Niranthin, a Lignan, Intercepts Leishmania donovani Repli- cation by Poisoning Topoisomerase of Parasite and Ameliorates Th1 Response to Cure Infected Mice" at the International Symposium on Challenges in Chemical Biology (ISCCB-2013) held at CSIR-Indian Institute of Chemical Biology, Kolkata, Indian Institute of Chemical Biology on January 27-29, 2013.Oral presentation:1. Selected for Oral Presentation for the best poster at "International Symposium on Modern Biology in 21st Century: Teaching and Research" held at University of Calcutta, Kolkata, India on December 7th and 8th, 2010. 2. Presented the work entitled "Elucidating the Mechanism of Resistance against Flavones in Leishmania donovani" at the 17th West Bengal State Science and Technology Congress held at Beleghata, Kolkata, India on 4th and 5th March, 2010.3. Presented the work entitiled "Potent lignans isolated from Phyllanthus amarus and Saraca indica inhibit Type IB Topoisomerase of Leishmania donovani and induces apoptotic like cell death" at the Frontiers in Chemical Biology on the Annual Meeting of Society of Biological Chemists (India) - Kolkata Chapter held at Sankarapur, West Bengal, India on April 22-24, 2011.