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Click here or scroll down to respond to this candidateCandidate's Name
PHONE NUMBER AVAILABLE EMAIL AVAILABLEPROFESSIONAL EXPERIENCELaboratory Operations ManagerVita Therapeutics, Baltimore MDAugust 2022 December 2023Thawed, expanded and sustained cell cultures from multiple cell lines of Induced Pluripotent stem cells (iPSCs)Harvested and cryopreserved iPSCs maintaining proper aseptic techniques.Differentiated iPSC cell cultures into Progenitor Cells while maintaining proper aseptic techniques.Performed and analyzed QC assays such as RNA extraction and qPCR and flow cytometry to check the levels of pax7 and CD271 in the cells.Scheduled PM services, operated, and calibrated laboratory equipment such as: biological safety cabinets, thermal cyclers, micro pipettes, -80Freezers, and incubators, to name a few.Delegated weekend responsibilities to people scheduled to work on weekends.Ordered and maintained inventory of laboratory supplies.Set up LN2 tank exchanges and maintained liquid nitrogen freezers.Maintained biohazardous liquid and dry waste disposal.Shipped out cells inside and outside of the country and received and updated laboratory deliveries.Laboratory ManagerJohns Hopkins University, School of Medicine, Pathology Department, Baltimore MDNovember 2021 August 2022Operated, maintained/calibrated and scheduled PM service of laboratory equipment such as: biological safety cabinets, thermal cyclers, micro pipettes, -80Freezers, -20Freezers, refrigerators, and incubators, to name a few.Updated and created laboratory procedures and protocols.Created in-house workorders.Maintained inventory of laboratory supplies.Created purchase orders and received invoices via SAP system.Set up and maintained biomedical and biohazardous waste disposal.Received all of the laboratory deliveries.PGT-M Laboratory TechnicianCooperGenomics, Livingston NJ,March 2020 October 2021Conducted Preimplantation Genetic Testing for Monogenic disorders (PGT-M) on single cell biopsies from embryos.Performed a variety of clinical tests including Sanger sequencing, Scalable, Fragile X CGG repeat testing, and other PCR reactions to detect diseases caused by mutations in genes such as FMR1, CFTR, SMN1, GJB2, RYR2, and HTTAnalyzed pedigrees and genetic reports to construct inheritance patterns of autosomal and X-linked disorders.Accessioned, received, extracted DNA from human specimens, measured product concentration, aliquoted, labeled and stored samples for clinical analysis.Maintained case logs, analysis sheets and documentation of assays in accordance with standard operating procedures.Troubleshot issues and maintained laboratory equipment and instruments required for tests.Infection Control Laboratory TechnicianMemorial Sloan Kettering Hospital, New York City, Infection Control DepartmentDecember 2018 February 2020Maintained an in-house access data system of organism surveillance.Updated and created laboratory procedures and protocols.Operated, maintained/calibrated and scheduled PM service of laboratory equipment such as: biological safety cabinet, PCR machine, gel imager, micro pipettes, -80Freezer, -20Freezer, refrigerator, and anaerobic chamber, to name a few.Created in-house workorders.Maintained inventory of laboratory supplies.Set up and maintained biomedical and biohazardous waste disposal.Setup and account with outside vendor for DNA sequencing service.Shipped biohazardous samples.Trained a new staff member in all official duties.Performed Multi-locus sequence typing (MLST) for Clostridioides difficile for identification on human samples.Plated C. diff for isolation on CC-BHIS plates in an anaerobic chamber; verified growth morphology with Prodisc test; Extracted DNA of selected colony; Performed PCR for 30 samples a week; gel electrophoresis for confirmation; sequence analysis of Sanger sequencing raw data.Analyzed Norovirus typing results.Trained in Pulse field gel electrophoresis (PFGE) testing for MRSA identification.QC Associate ChemistThermo Fisher, Fair Lawn, NJ Quality Control Dry Chemistry DepartmentJuly 2018 November 2018Performed analysis on environmental dry chemical samples as required, while maintaining full compliance with all applicable US FDA cGMP regulations and internal Standard Operating Procedures (SOP). Observed all health and safety rules and policies of the company.Maintained laboratory records, at real time, related to all testing activities assuring that they are accurate and up to date.Utilized techniques of wet chemistry tests, such as titration, dissolution, turbidity, colorimetric, particle/mesh size computing, water content determination, melting point, specific rotation, atomic absorbance detection, in accordance with the USP/NF, FCC, EP, BP, ACS or JP compendia.Operated laboratory equipment such as conductivity meter, pH meter, Kjeldahl distillation unit, oven, Karl Fischer, FAAS, FT/IR, UV, auto titrator, polarimeter, to name a few.Wet ChemistSGS North America Inc, Fairfield, NJ Chemistry DepartmentFebruary 2018 April 2018Performed analysis on environmental samples as required, while maintaining full compliance with all applicable US FDA cGMP regulations and internal Standard Operating Procedures (SOP). Observed all health and safety rules and policies of the company.Maintained laboratory records, at real time, related to all testing activities assuring that they are accurate and up to date.Performed laboratory inventory and maintenance and purification of supplies, as well as the maintenance of the laboratory environment.Utilized techniques of wet chemistry tests, such as titration, particle size computing, insoluble residue determination, moisture vapor permeability and analytical identification testing of raw material, in accordance with the USP, EP, ACS or JP compendia.Operated and maintained/calibrated laboratory equipment such as conductivity meter, pH meter, autoclave, oven, centrifuge, spectrometer, to name a few.Scientist IAmneal Pharmaceuticals, Piscataway, NJ Research and Development Department March 2017June 2017Performed quantitative analysis for pharmaceutical drug-substances, excipients and drug products using USP/NF, EP, in-house test methods and standard protocols, while maintaining full compliance with all applicable US FDA cGMP regulations and internal Standard Operating Procedures (SOP).Maintained laboratory records related to all testing activities assuring that they are accurate and up to date.Operated and maintained/calibrated laboratory equipment as per manufacturers recommendations and companys Preventive Maintenance program.Utilized techniques of Spectrophotometry, GC, dissolution, titration and wet chemistry tests, such as LOD, ROI, viscosity, detection of heavy metals and assay, in accordance with the USP.Lab Research AssistantAlexandria Center for Life Science, New York City, NY, Dr. Thomas Wisniewski LabJanuary 2013 May 2015Studied a therapeutic approach of utilizing the immune system to reduce amyloid beta burden in the brains of Alzheimers Disease models.Assisted in various laboratory experiments including, immunohistochemistry, immunofluorescence, as well as quantitative, and statistical analysis.EDUCATIONNew York University, New York City, NYM.S. Biology 2015Thesis: The Effect of Genomics on a Better Understanding of the Pathogenesis of TB inside alveolar macrophages (AMs).Coursework: Virology, Genomics and Global Health, Infectious Diseases, Programing for BiologistsFerrum College, Ferrum, VAB.S. Biology and Pre-Professional Sciences 2008Areas of Concentration: Pre- MedicineMinor: ChemistryThesis: The Presence of E. coli in Local Tap Water Through Colilert Testing.Coursework: Microbiology, Cell Biology, Organic Chemistry I &II, GeneticsACADEMIC EXPERIENCENYU Molecular Biology Lab Dr. Nikolai Kirov LabIdentifying a possible homolog of Brk gene in cricket genome, Fall 2014Isolated and purified DNA from E. coli culture.Performed size exclusion isolation of DNA, quantification, PCR amplification, and hybridization of DNA. Also conducted Southern blots.Conducted a comparison analysis of the cricket and drosophila genome at the Brk gene region.Implemented Southern Blot Test for the detection of sequence in cricket DNA with a similar function to the Brk gene.Ligated possible homolog into bacterial vectors that were incubated in the LB medium, used the blue-white selection process, used T7 sequence to sequence the DNA on the sequencing gel.Performed BLASTN analysis on sequencing data.In vitro mutagenesis of a Brk gene with BstB1 and Pvu1 site mutations, Spring 2015Cloned Brk gene in plasmid and executed an extension overlap mutagenesis on F48R and R82S sites.Utilized Xl-1 cells to run PCR reactions on complimentary outside primers and inside mutant primers which to yield clones with blunt vectors.extracted recombinant proteins from cultured cells.Conducted Western Blot to determine the molecular weight of the antigen.OTHER EXPERIENCEBookkeeperFrazier Industrial Inc. April 2016March 2017Provide clerical and administrative support to management by coordinating payroll, placing office orders for supplies, and scheduling maintenance for machines and/or equipment.Create financial transactions and reports by making purchase orders and requisitions.ReceptionistC.A. Shea & Company Inc. Spring 2012 Spring 2013Performed administrative duties and handled U.S. Custom bonds by archiving, filing, and approving bond terminations and riders.Customer Service RepresentativeFauzi Azar Hostel, Israel Fall 2011 Winter 2011Served as the first point of contact for the company by greeting customers, checking guests in and out, and providing housekeeping services.Provided guided informative tours in Nazareth.ReceptionistRandolph Medical & Renal Associates Spring 2011 Fall 2011Provided clerical support in a medical office by answering phones, and scheduling appointments for various medical tests.Acted as liaison for hospitals and laboratory facilities regarding testing results and medical information. |
