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Click here or scroll down to respond to this candidateCandidate's Name
ORCID: https://orcid.org/Street Address -0001-5458-1099PHONE NUMBER AVAILABLEEMAIL AVAILABLEObjectiveIm a postdoctoral fellow in Dr. Kian-Huat Lims lab research on therapeutic targets for pancreatic cancer. My interest in research started before pharmacy school and continued on during pharmacy school. Through researching in the lab, I have been able to learn various techniques. I am planning to pursue academia as I feel that there are many students that could become scientists but do not have the opportunity to do so. EducationDoctorate of Philosophy-Chemistry : 09/2019-12/2023 University of Manitoba, Winnipeg, MBVarious techniques include NMR, IR, mass spectrometry, culturing, and structure elucidation. Doctorate of Pharmacy : 08/2012- 05/2019University of Louisiana at Monroe, Monroe, LAVarious training courses including:Immunization certification through APhA, certification for use of vivarium, NMR course (graduate course audit)Work ExperiencePostdoctoral Fellow: 10/2023-presentWashington University at St. Louis, MOI am working on trying to get a TPL2 KO cell line to inject into the mice. Additionally, I have conducted genotyping and breeding of mice to obtain a KPPC-TPL2-KO mice to investigate the mechanism of TPL2. During this time I have done other molecular biology techniques in addition to this. Research Assistant: 09/2019-09/23University of Manitoba-Winnipeg, MBI am responsible for learning various techniques to do structure elucidation (NMR, IR, mass spectrometry). Also, Im working on the usnic acid biosynthesis pathway in lichens and trying to have functional expression of the lichen genes outside of lichens.Teaching Assistant: 09/2019-05/2022University of Manitoba-Winnipeg, MBI am responsible for teaching students in the organic chemistry lab (CHEM 2210, 2220, 2122). I assist students in their experiments and help them understand why various techniques are necessary to learn. Pharmacy Intern: 07/2018 05/2019Walgreens- Monroe, West Monroe, LAI am responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, provide MTM services, transfer prescriptions between pharmacies, and give immunizations to patients. Ive also helped patients who were in buyout pharmacies transition into the Walgreens system. Pharmacy Intern: 08/2016-07/2018Rite Aid-Monroe, West Monroe, Ruston, LAI was responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, provide MTM services, transfer prescriptions between pharmacies. Ive also aided in closing of Rite Aid pharmacies and with transitioning Rite Aid pharmacies to Walgreens during the buyout. Pharmacy Intern: 07/2016-03/2017Walmart, Rayville, LAI was responsible for inputting prescriptions, dispensing medications to patients, counsel patients on medications, and manage inventory.Research ExperienceWashington University at St. Louis, MO (10/2023-present) 1. To understand the TPL2 mechanism in PDACa. KO using CRIPSR-CAS9b. Breeding mice to achieve KPPC-TPL2c. If a drug (tilpisertib) in the clinic is worth pursuing as a therapy option for PDAC. 2. Codinga. Single cell analysis in Rb. Some basics in PythonUniversity of Manitoba-Winnipeg, MB (09/2019-09/2023) 3. Evaluating different lichens in the herbarium on campus. Made crude extracts of lichens and isolated lichen secondary metabolites from extraction from lichens to using column chromatography to isolate metabolites. Metabolites were identified through TLC plates, NMR spectroscopy, and mass spectroscopy.4. Evaluating protein structure of polyketide synthase domains and understanding the difference between different species5. Managing collaboration projects with groups in both New York and Newfoundland. ULM-College of Pharmacy- Monroe, LA (03/2014-07/2019) 6. Collection of lichens from the field (Russell Sage Wildlife Refugee) and identification of lichens 7. Made crude extracts of lichens and isolated lichen secondary metabolites from extraction from lichens to using column chromatography to isolate metabolites. Metabolites were identified through TLC plates, NMR spectroscopy, and mass spectroscopy. 8. Biological assays were conducted to test metabolites through MTT assay, wound healing assay, invasion assay, and western blot assay.9. Current project is isolation of fungi from lichens and seeing if they could keep the integrity and produce the same metabolitesSkillso Software Skills: Word, Excel, Powerpoint, Schrodinger molecular modelling, Coding (R, Python) o Molecular Biology: Cloning, DNA isolation and purification, RNA isolation and purification, protein isolation and purificationo Analytical Technique: NMR, Thin Layer Chromatography, High-Performance Liquid Chromatography(HPLC), Liquid Chromatography Mass Spectrometry (LC-MS) o In Vitro Studies: MTT, wound healing, invasion assays o Identification technique: identification of lichen and fungi through an identification key o Extraction of natural products through varying techniques PublicationJiang, Y., Rex, D.A.B., Schuster, D., Neely, B.A., Rosano, G.L., Volkmar, N., Momenzadeh, A., Peters-Clarke, T.M., Egbert, S.B., Kreimer, S. and Doud, E.H., 2023. Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry. arXiv preprint arXiv:2311.07791. Covington, E.W., Jasper Trotter, S.L., Arnold, R.D., Amin, R., Egbert, S. and Chung, A., 2023. Prospective pilot study evaluating a vitamin D3 loading dose in critically ill children with vitamin D deficiency. Fundamental & Clinical Pharmacology.Emily G. McDonald, MD MSc, Gloria Aggrey, MD, Abdullah Tark Aslan, MD, Michael Casias, PharmD, BCIDP, AAHIVP, Nicolas Cortes-Penfield, MD, Mei Qin (Denise) Dong, Pharm.D., Candidate's Name , S., Hoffman, J. R., McMullin, R. T., Lendemer, J. C., & Sorensen, J. L. (2022). Unraveling usnic acid: A comparison of biosynthetic gene clusters between two reindeer lichen (Cladonia rangiferina and C. uncialis). Fungal Biology.Terlouw, B. R., Blin, K., Navarro-Muoz, J. C., Avalon, N. E., Chevrette, M. G., Egbert, S., ... & Medema, M. H. (2022). MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Research.Patient and Herbal SupplementFebruary 2018-Drug Topics articlehttps://www.drugtopics.com/view/patients-and-herbal-supplements Ebrahim HY, Elsayed HE, Mohyeldin MM, Akl MR, Bhattacharjee J, Egbert S, El Sayed KA. Norstictic Acid Inhibits Breast Cancer Cell Proliferation, Migration, Invasion, and In Vivo Invasive Growth Through Targeting C-Met. Phytother Res. 2016 Apr;30(4):557-66. doi: 10.1002/ptr.5551. Epub 2016 Jan 6. PubMed PMID: 26744260; PubMed Central PMCID: PMC5045260.o Extraction of natural products through varying techniques PresentationInvestigation of the undiscovered potential for lichen-fungi metabolites (poster) July 2023 ASPInvestigation of the undiscovered potential for lichen-fungi metabolites (poster) May 2023 Ft. Detrick Spring Research FestivalOutstanding Poster Award in Applied and Environmental Biology Lichens, an underexplored symbiotic organism, issues in literature, and future potentials. (oral) March 2023 CanFunNetInvestigation of the undiscovered potential for lichen-fungi metabolites (poster) February 2023 NIH Graduate Student SymposiumA New Perspective On Usnic Acid Biosynthesis In Cladonia Rangiferina (oral) Sept 5, 2022 ICPSIdentifying the Squamatic Acid Biosynthetic Gene Cluster in Lichen Cladonia uncialis (poster) July 2022 ASPIdentifying the Squamatic Acid Biosynthetic Gene Cluster in Lichen Cladonia uncialis (oral) June 3, 2022 CanFunNetA New Perspective on Usnic Acid Biosynthesis in Cladonia rangiferina (poster) October 18-21, 2021 MANA 2021A New Perspective on Usnic Acid Biosynthesis in Cladonia rangiferina (poster) July 16, 2021 ASPWhat do we really understand about lichen biosynthetic gene cluster (oral) May 26, 2021 MSAhttps://www.youtube.com/watch?v=yHUCPw1q-jcLichen-Past, Present Future (oral)May 4, 2021 Torrey Botanical SocietyJudgingUndergraduate medical student research programAugust 12, 2021 University of Manitoba-Faculty of Medicine 3MT competition (undergraduate)August 10, 2021 University of Manitoba-Faculty of Science Extracurricular Activities/OrganizationsLOGIC (WIC) retreat 2022TBAPosition held: co-chairCCCE 2021August 15-21, 2021Position held: volunteerLOGIC (WIC) retreat 2021August 7-13, 2021Position held: organizerPhD BalanceJune 2021-presentStronger Together Team leader (August 2021-Dec 2021) Vice President (October 2021-Dec 2021)President (Jan 2022- )American Pharmacist Association-Academy of Pharmaceutical Research and Science (APhA-APRS) June 2021-presentCommittee memberUniversity of Manitoba International Student Association (UMISO) May 2021-presentVice President LogisticsFemales in Mass Spectrometry (FeMS) Mentoring Program April 2021-presentPlanning committeeSummer project supervisorMay 2020-August 2020Supervised an undergraduate student researching the synthesis of lichen secondary metabolites. U of M Womyns CentreJuly 2020-May 2021Vice President Social MediaLOGIC (WIC) retreat 2020May 17-23, 2020Position held: organizerCOVID-19 translationApril 2020-May 2020Science Fair Project coordinatorJan 2020-March 2020Supervised elementary students with their science fair project. This involved looking into the bioactive compounds of cinnamon and testing its activity against pathogenic bacteria. CWIC (Canadian Working Towards Inclusivity in Chemistry) Jan 2020-presentIncoming CFO (May 2021-present)Women in Chemistry/Working Towards Inclusivity in Chemistry (WIC) January 2020-PresentCo-President (Internal May 2021-present)Co-Vice president Recruitment (July 2020- May 2021) U of M Chemistry Graduate AssociationSeptember 2019-PresentULM Student Research ClubJanuary 2018-May 2019Positions held: Vice President, PresidentULM Global AmbassadorMay 2016-May 2019Positions held: Vice President, President, Grad Liaison Events Held: Health Fair, Art Crawl, Orientation for International Students ULM Student Research SymposiumMarch 2016, March 2017, April 2018ULM APhA-ASPAugust 2015-May 2019ULM American Chemistry SocietyJanuary 2014-May 2015Position held: HistorianULM Pre-Pharmacy OrganizationJanuary 2013-May 2015ULM Honors ProgramAugust 2012-May 2019Positions held: Freshmen Representative, Treasurer, President, Grad Liaison. Thesis defense: May 2015Awards: Student of the Year (two years in a row), Freshmen of the Year Training2021 VADA Program Data Science and AI Summer School July 13-15, 2021Reviewing2021 CADTH SymposiumJuly 2021Abstract ReviewerUMGSA awardsSummer 2021 |
