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| | Click here or scroll down to respond to this candidateEducation:Northeastern University Boston, MA Masters Degree in Chemical Engineering: GPA 3.Street Address Sept 2018-Dec 2020Rutgers The State University of New Jersey New Brunswick NJ Bachelors Degree in Chemical Engineering: GPA 3.Street Address Sept 2012-Oct 2016Work ExperienceSenior Research Associate Decibel Therapeutics Jan 2023 Feb 2024 (LNP) Lipid nanoparticle particle transfection, AAV transduction, RNAdvance isolation with RT-qPCR analysisHEK293T, HEK293A, Hela cell culture, SAM D73 crispr cell line maintenance, automated fluorescence counting with Sony Cell Sorter, Guava Flow Cytometry for high throughput potency assay, ELISA for potency assays, Enzymatic Assays, Gel Electrophoresis for Western Blotting, tissue cultureBenchling plasmid check in and design, plasmid dilution, promotor expression, dirty AAV preps from cell lysates and supernatants, Recording experimental protocols and data on electronic lab notebook (ELN)Method development for functional assay for hearing disorder programs of pendrin, stereocilin, and otoferlinScientist I Axcella Therapeutics Nov 2022 Dec 2022Human Lung Microvascular Endothelial Cell culture, CTF reading, RNA Isolation, DAPI cell stain countingELISA based testing for markers for NASH and long covid, PCR, DNA preparation for molecular biologyTryzol lysis with zymo kit based RNA isolation, electronic multichannel micropipetteScientist I Thermofisher Scientific Jan 2022 Oct 2022 qPCR, QuantStudio Flex 7, cell free DNA isolation, Tumor Cell Culture, IonTorrent sequencingKing Fischer automated DNA isolation, E.coli transformation, Midi-Prep Kit Isolation, Nuclei Acid SynthesisScientist I - Sanofi Mar 2021 Dec 2021 Immunological Assays such as MSD, ELISA, ELISpot, flow cytometry and RT-qPCRFlow cytometry with antibody fluorophores for cell sorting and quantification (FITC, BUV395, PE, PE/Cy7, etc)Radiation pulsing with tritium thymine to measure cell proliferation, In vitro ImmunofluorescenceT cell isolation, transfection, and culturing, CD3 stimulation, CHO TCRa stimulation, moDC isolation, differentiation, and T cell coculture, tissue culture, immunochemistrySplenocyte mice extraction and culturing, Cardiac blood mice extraction and culturing. Mouse CO2 euthanasiaSmall molecule dosing assay with IgG and IgM antibodies for immune checkpoint markers, LNP encapsulated RNA transfection of immune cellsResearch Student (Retinal Regeneration) - Northeastern University Nov 2018 Mar 2021 Morphology DAPI and Alexfluoro staining to qualify cell differentiation within different hydrogels conjugated to small molecules, quantitative image analysisCCK spectroscopy, human retinal progenitor cell culturing and IPSC differentiation.Hydrogel conjugation to small molecules like RGD and YSIR via oxidationSkillsPrograms: MATLAB, Simulink, Maple, SolidWorks 2017, LIMS software, Benchling, PRISM, FlowJo, ImageJLaboratory Procedures: Biological Safety Level 2, GMP stem cell culturing, plasmid transfection, Cytometry, ELISA, Tritium PulsingInstrumentation: Fortessa Flow Cytometer, Sony cell sorter, QuantStudio PCR, Electronic Micropipetes, KingFischer DNA isolator, Incucyte,ReferencesRebecca Carrier, Vijay Patel, Hemax Patel, Shishir Chundawat |