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Candidate's Name
EMAIL AVAILABLE, EMAIL AVAILABLE PHONE NUMBER AVAILABLEEDUCATIONTokyo Metropolitan University, Japan Oct Street Address -March 2024PhD, Evolutionary Genetics. D-5.Banasthali Vidyapith University, India June Street Address -May 2015Master of Science, Bioinformatics.Mahatma Jyotiba Phule Rohilkhand University June 2010- May 2013Bachelor of Science, Biological Science.WORK EXPERIENCETemple University (IGEM), PA-USA. July 2023-PresentVisiting facultyBenchmarking methods on scRNA-seq, scATAC-seq, and single-cell omics sequencing.University of Pennsylvania (Penn Vet), PA-USA. Dec 2022-July 2023Visiting Research ScholarSingle cell transcription analysis.Tokyo Metropolitan University, Japan. Oct 2018-Oct 2022Research Assistant (Evolutionary Genetics laboratory)Evolution of gene expression dynamics in response to cold in Drosophila albomicans.Senior Teaching Assistance (Evolutionary Genetics laboratory)Course: Next Generation Sequencing and Data Analysis.Course: Perl programming and analyzing image data.Teaching Assistants (Laboratory of Animal Ecology)Course: PC maintenance and updating of software (R, ImageJ, etc.).CSIR-NBRI (National Botanical Research Institute), India. Mar 2016-April 2018Project Assistant level-2 (Computational Plant and Molecular Biology lab)Handled and analyzed Next-Generation Sequencing data.PublicationCandidate's Name , Sakshi Srivastava, Divya Srivastava, and Meetali Sinha (2016) Insilico identification of novel compound against Urinary Tract Infection Int. Res. J. Biological Sci., 5(3), 51-55.Dhar, Yogeshwar V., Deepika Lakhwani, Ashutosh Pandey, Candidate's Name , Prabodh K. Trivedi, and Mehar H. Asif. "Genome-wide identification and interactome analysis of members of two-component system in Banana." BMC Genomics 20, no. 1 (2019): 1-15.Khare, Ria, Yogeshwar V. Dhar, Gurpreet Sandhu, Candidate's Name , Smita Kumar, Aruba Khan, Mehar Hasan Asif, and Prabodh Kumar Trivedi. "Genome-wide expression and variation in nucleotide sequences lead to differential response of Arabidopsis thaliana ecotypes towards arsenic stress under sulfur limiting condition." Environmental and Experimental Botany 195 (2022): 104764.Kumar, Vinod, Deepika Singh, Adity Majee, Candidate's Name , Mehar Hasan Asif, Aniruddha P. Sane, and Vidhu A. Sane. "Identification of tomato root growth regulatory genes and transcription factors through comparative transcriptomic profiling of different tissues." Physiology and Molecular Biology of Plants 27, no. 6 (2021): 1173-1189.In Lakhwani, Deepika, Yogeshwar Vikarm Dhar, Candidate's Name , Ashutosh Pandey, Prabodh Kumar Trivedi, and Mehar Hasan Asif. "Genome wide identification of MADS box gene family in Musa balbisiana and their divergence during evolution." Gene (2022): 146666.SKILLSBioinformatic:Language: R, Basic PERL programmingSingle cell RNA-Seq, scATAC-Seq, SNV calling in scRNA-Seq and bulk, Denovo genome and reference-based transcriptome assembly(Cufflinks, RSEM, Trinity), read mapping(STAR, Bowtie, TopHat), alignment(Clustal Omega, Clustalx, Tcoffee, BLAST), comparative genomics and transcriptomics, differential gene expression (EdgeR, Cuffdiff, NOIseq), functional annotation, data analyses and Clustering using R, MeV and various data visualization through R, network analyses using Cytoscape, quality trimming, tree topology comparison, Genome-wide identification of gene families, genome-wide SNP analyses, Pymol, Chimera, Autodock, Chemdraw, Sequence, and expression distance using megacc.Technical (Wet Lab):Hands-on expertise in basic molecular biology and Next generation sequencing practices like DNA/RNA extraction and quality analysis, PCR and Gel-electrophoresis, Library Preparation using nanovue, PureLink mini kit, Bioanalyser-2100 Agilent RNA 6000, Bioanalyzer-2100 High Sensitivity DNA kit, and TapeStation-2200 High Sensitivity DNA Analysis, Qubit dsDNA High sensitivity, TruSeq Stranded mRNA Library Preparation. primer design, CRISPR/Cas9 design gene editing in mammalian cell culture.PhD ProjectTopic: Evolution of gene expression dynamics in response to cold in Drosophila albomicans.Objective: Major objective of my current work is to find genes responsible for cold acclimation similar phenotypes and how species genetically modify for their adaptation to tropical to temperate regions within species.