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1CURRICULUM VITAEXin WuOncoResponse  1124 Columbia Street Suite 300 Seattle WA  Cell PHONE NUMBER AVAILABLE  EMAIL AVAILABLE QUALIFICATION SUMMARYBoth academia and industry experience with strong cancer and immunology knowledge and practical bench work experienceAdept in project design, result analysis and problem solvingExpert in molecular biology and virus-based gene therapyExtensive experience in cancer biomarker screening and related cell signaling pathway analysisExperience in animal husbandry including knock-in and knock-out mice colony maintenance and histology study.Skilled in immunotyping different immunological cell populations from various types of human tissues including whole blood, bone marrow, cerebrospinal fluid, ascites and cancer tissues using multicolor flow cytometry and FlowJoDesign gene functional study using siRNA and CRISPR-Cas9 (both knock-in and knock-out)Antibody protein purification using AKTA Explorer, Avant and Pure, Agilent 1260 Infinity HPLC as well as Waters ACQUITY Premier UPLC SystemExperience in bioinformatic analysis including TCGA dataset, microarray, RNA-seq, Linux, R, C++ programmingWORKING EXPERIENCEJanuary 2021  present Associate Scientist Oncoresponse LLCCharacterize antibody specificity by flow cytometry, ELISA, alphaLISA, octet RH16Establish stable gene expression cell line using flow cytometryCharacterize biophysical property of antibody using DSF, IEF, endotoxin quantification, reducing and non-reducing SDS PAGEProtein-protein interaction using magnetic beads immunoprecipitation and epitope mapping using Western blotCRISPR gene knockout Fut8 geneConstruct expression vectors and use HEK293 6e and EpiCHO cells for monoclonal antibody productionSmall scale monoclonal antibody production and purification using AKTA, and UPLC System November 2018  October 2019 Technologist Bloodworks Northwest, Seattle, WAAnalysis of CD34 stem cells in whole blood and apheresis of autologous transplant cancer patients as well as cord blood by flow cytometryEstablish the protocol of lymphocytes analysis using CD3/CD4/CD8/CD45 by flow cytometryDiagnose hematological diseases such as leukemia and lymphoma using flow cytometry January 2015  March 2017. Postdoctoral Fellow, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Takeshi KuritaExplore the interaction between tumor and its microenvironment in human uterus leiomyomasStudy the leiomyoma growth under the regulation of hormone by PDTX mouse modelEstablish MED12 knock-in mouse model by CRISPR to study the leiomyoma growth regulation October 2009  January 2015. Research Associate, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Gregory OttersonExplore PRMT5 related signaling pathways in drug resistance in lung cancer chemotherapy 2Explore the effect of small molecules targeting PRMT5 as a new class of drug in treating lung cancer, GBM and hematologic malignanciesDetermine the effect of microRNA on lung cancer pathogenesis October 2004  October 2009. Postdoctoral Researcher, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Gregory OttersonIdentify microRNA biomarkers for the diagnosis and treatment of lung cancerDesign in vivo system to screen the microRNA targetsDetermine the effect of drugs on the microRNA profiling October 1996  September 2004. Graduate Fellow and Investigator, Liver Cancer Institute, Fudan University, Shanghai, ChinaIdentify new tumor suppressor gene on chromosome 8p in human liver cancerEstablish a new human primary liver cancer cell lineSupervise the laboratory and provide the experimental instruction for the graduate students and visiting scholarsEDUCATIONPh.D, Biochemistry & Molecular biology. Fudan University, Shanghai, China. January 2003Dissertation: Screening Candidate Tumor Suppressor Gene on Chromosome 8p in Human Hepatocellular CarcinomaMS, Biochemistry. Shanghai Medical University, Shanghai, China. July 1996Dissertation: Effects on the cytokine expression of mouse macrophages by exogenous gangliosidesMD, Clinical Medicine. Bengbu Medical College, Bengbu, Anhui, China. July 1991 CONTINUED EDUCATIONDepartment of Computer and Information Science. The Ohio State University. Sep. 2010- July 2015 Computer Science major (undergraduate)CERTIFICATEAABB Cellular Therapies Certificate Program 2020Integrative Statistical Analysis of Genome Scale Data. Cold Spring Harbor laboratory, NY, 2009 PUBLICATIONS1. Xin Wu, Vanida Serna, Justin Thomas, Wenan Qiang, Michael Blumenfeld, and Takeshi Kurita. Subtype- specific contribution of tumor-associated fibroblasts to the pathogenesis of uterine leiomyoma. Cancer Research. 2017 Dec 15;77(24):6891-6901.2. Vanida Serna, Xin Wu, Justin Thomas, Wenan Qiang, Michael Blumenfeld, and Takeshi Kurita. Cellular kinetics of MED12 mutant uterine leiomyoma growth and regression in vivo. Endocrine-Related Cancer. 2018 Jul;25(7):747-759. .3. Yan F, Alinari L, Lustberg ME, Martin LK, Cordero-Nieves HM, Banasavadi-Siddegowda Y, Virk S, Barnholtz-Sloan J, Bell EH, Wojton J, Jacob NK, Chakravarti A, Nowicki MO, Wu X, Lapalombella R, Datta J, Yu B, Gordon K, Haseley A, Patton JT, Smith PL, Ryu J, Zhang X, Mo X, Marcucci G, Nuovo G, Kwon CH, Byrd JC, Chiocca EA, Li C, Sif S, Jacob S, Lawler S, Kaur B, Baiocchi RA. Genetic validation of the Protein Arginine Methyltransferase PRMT5 as a candidate therapeutic target in glioblastoma. Cancer Res. 2014;74(6):1752-65.34. Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors. Diagn Pathol. 2013;8:201. 5. Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sf S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB. PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27 (Kip1). PLoS One. 2013;8(9):e74710. 6. Duan W, Gao L, Zhao W, Leon M, Sadee W, Webb A, Resnick K, Wu X, Ramaswamy B, Cohn DE, Shapiro C, Andreassen PR, Otterson GA, Villalona-Calero MA. Assessment of FANCD2 nuclear foci formation in paraffin-embedded tumors: a potential patient-enrichment strategy for treatment with DNA interstrand crosslinking agents. Transl Res. 2013;161(3):156-64. 7. Mortazavi A, Ling Y, Martin LK, Wei L, Phelps MA, Liu Z, Harper EJ, Ivy SP, Wu X, Zhou BS, Liu X, Deam D, Monk JP, Hicks WJ, Yen Y, Otterson GA, Grever MR, Bekaii-Saab T. A phase I study of prolonged infusion of triapine in combination with fixed dose rate gemcitabine in patients with advanced solid tumors. Invest New Drugs. 2013;31(3):685-95. 8. Martin LK, Grecula J, Jia G, Wei L, Yang X, Otterson GA, Wu X, Harper E, Kefauver C, Zhou BS, Yen Y, Bloomston M, Knopp M, Ivy SP, Grever M, Bekaii-Saab T. A dose escalation and pharmacodynamic study of triapine and radiation in patients with locally advanced pancreas cancer. Int J Radiat Oncol Biol Phys. 2012;84(4):e475-81.9. Yan F, Wu X, Crawford M, Duan W, Wilding EE, Gao L, Nana-Sinkam SP, Villalona-Calero MA, Baiocchi RA, Otterson GA.The search for an optimal DNA, RNA, and protein detection by in situ hybridization, immunohistochemistry, and solution-based methods. Methods. 2010; 52(4):281-6. 10. Nuovo GJ, Wu X, Volinia S, Yan F, di Leva G, Chin N, Nicol AF, Jiang J, Otterson G, Schmittgen TD, Croce C. Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol. 2010;19(3):135-43. 11. Duan W, Gao L, Wu X, Wang L, Nana-Sinkam SP, Otterson GA, Villalona-Calero MA. MicroRNA-34a is an important component of PRIMA-1 induced apoptotic network in human lung cancer cells. Int J Cancer. 2010; 127(2):313-20.Kosuri KV, Wu X, Wang L, Villalona-Calero MA, Otterson GA. An epigenetic mechanism for capecitabine resistance in mesothelioma. Biochem Biophys Res Commun. 2010; 391 (3) :1465-70.12. Wu X, Piper-Hunter MG, Crawford M, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. MicroRNAs in the Pathogenesis of Lung Cancer. Journal of Thoracic Oncology. 2009; (8):1028-34. 13. Crawford M, Batte K, Yu L, Wu X, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. MicroRNA 133B targets pro-survival molecules MCL-1 and BCL2L2 in lung cancer. Biochem Biophys Res Commun. 2009;388(3):483-9.14. Duan W, Gao L, Wu X, Hade EM, Gao JX, Ding H, Barsky SH, Otterson GA, Villalona-Calero MA. Expression of a mutant p53 results in an age-related demographic shift in spontaneous lung tumor formation in transgenic mice. PLoS One. 2009;4(5):e5563. 15. Chai G, Li L, Zhou W, Wu L, Zhao Y, Wang D, Lu S, Yu Y, Wang H, McNutt MA, Hu YG, Chen Y, Yang Y, Wu X, Otterson GA, Zhu WG. HDAC inhibitors act with 5-aza-2'-deoxycytidine to inhibit cell proliferation by suppressing removal of incorporated abases in lung cancer cells. PLoS ONE. 2008;3(6):e244516. Wu X, Jia HL, Wang YF, Ren N, Ye QH, Sun HC, Wang L, Liu YK, Tang ZY, Qin LX. HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma. Oncogene. 2006; 25(12):1832-4017. Duan W, Gao L, Wu X, Zhang Y, Otterson GA, Villalona-Calero MA. Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells. Exp Cell Res. 2006;312(17):3370-8.18. Zhang H, Ye QH, Ren N, Zhao L, Wang YF, Wu X, Sun HC, Wang L, Zhang BH, Liu YK, Tang ZY, Qin LX. The prognostic significance of preoperative plasma levels of osteopontin in patients with hepatocellular carcinoma. J Cancer Res Clin Oncol. 2006;132(11):709-17. 19. Hui L, Zhang X, Wu X, Lin Z, Wang Q, Li Y, Hu G. Identification of alternatively spliced mRNA variants related to cancers by genome-wide ESTs alignment. Oncogene. 2004; 23 (17):3013-23 420. Li Y, Tian B, Yang J, Zhao L, Wu X, Ye SL, Liu YK, Tang ZY. Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics. J Cancer Res Clin Oncol. 2004;130(8):460-8. 21. Zhao L, Qin LX, Ye QH, Zhu XQ, Zhang H, Wu X, Chen J, Liu YK, Tang ZY. KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma--a functional analysis. J Cancer Res Clin Oncol. 2004;130 (12):719-27.22. Jiang Y, Zhou XD, Liu YK, Huang XW, Zhao Y, Xue Q, Sun RX, Chen J, Wu X. Antisense Tcf inhibits the neoplastic growth of liver cancer cells. J Cancer Res Clin Oncol. 2004;130(11):671-8 23. Liang CM; Zhong CP; Zheng N; Wu X; Sun RX; Liu YK; Ye SL To construct a recombinant adeno- associated virus vector carrying the gene encoding the mature peptide of secondary lymphoid-tissue chemokine. Acta Anatomica Sinica, 2004; 35(2):147-51 24. Wu X, Jia HL, Liu YK, Li Y, Chen J, Zhao Y, Yu L, Qin LX, Tang ZY. Comparative study on gene expression difference between high and low metastatic human hepatocellular carcinoma cell strains. China Oncology, 2003(2); 13:97-10525. Huang R, Xing Z, Luan Z, Wu T, Wu X, Hu G. A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas. Cancer Res. 2003; 63(13): 3775- 8226. Zhang LH, Qin LX, Ma ZC, Ye SL, Liu YK, Ye QH, Wu X, Huang W, Tang ZY. Allelic imbalance regions on chromosomes 8p, 17p and 19p related to metastasis of hepatocellular carcinoma: comparison between matched primary and metastatic lesions in 22 patients by genome-wide microsatellite analysis. J Cancer Res Clin Oncol. 2003;129:279-8627. Jiang Y, Zhou XD, Liu YK, Wu X, Huang XW. Association of hTcf-4 gene expression and mutation with clinicopathological characteristics of hepatocellular carcinoma. World J Gastroenterol, 2002;8: 804-7 28. Wang YC, Chen ZR, Chen ZW, Guan XQ, Song HY, Wu X, Liu YK. Recombinant human heparin binding neurite promoting factor expressed with yeast stimulates neuritis outgrowth. Chinical Medical J, 2002; 115(9):1352-729. Jiang Y, Zhou XD, Liu YK, Wu X. T cell factor 4 gene expression and mutation in hepatocellular carcinoma. China Oncology, 2002; 12(1): 5-830. Jiang Y, Zhou XD, Xue Q, Liu YK, Wu X. Relationship between hTcf-4 gene expression and liver cancer metastasis. Shaanxi Oncology Medicine, 2002; 10 (2): 88-90 31. Jiang Y, Zhou XD, Liu YK, Wu X. Study on the expression and mutation of hTcf-4 gene in liver cancer cell lines. Chinese Journal of Experimental Surgery, 2002; 19 (2): 107-9 32. Xu L, Hui L, Wang S, Gong J, Jin Y, Wang Y, Ji Y, Wu X, Han Z, Hu G. Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma. Cancer Res. 2001 Apr 1; 61(7):3176-8133. Wang YC, Chen ZR, Chen ZW, Gong XQ, Song HY, Wu X, Liu YK. Recombinant human heparin- binding neurite-promoting factor expressed with yeast and its neurite-promoting activity. Chinese Journal of Traumatology, 2001; 17(12):740-434. Zhang W, Zhou JP, Liu YK, Wu X. Expression of leukemia inhibitory factor mRNA and its localization in the endometrium of early pregnant mouse. J. Shanghai Medical University, 2000;27(4):280-2 35. Zhang JH, Wu JP, Liu YK, Zhou JP, Wu X. Changes of Endometrial _3 Integrin during Early Pregnancy. Reproduction and Contraception, 2000; 20(3):151-536. Liu YK, Zhang CD, He B, Wu X, Ye SL, Tang ZY. E-selectin mediates early adhesion of HepG2 hepatoma cells to endothelial cells. Chin J Oncol, 2000; 22(6):449-52 37. Wu X, Liu YK, Zhang XY. Effects on the cytokine expression of mouse macrophages by exogenous gangliosides. China Oncology, 1999; 9(2):138-14038. Jiang YA, Wu X, Liu YK. The detection of telomerase activity in hepatocarcinoma. China Oncology, 1999; 9(4): 272-539. Zhu J, Zhou JP, Liu YK, Wu X. Expression of Intercellular Adhesion Molecule 1 mRNA In the Mouse's Endometrium During Implantation Periods. Journal of Shanghai Medical University, 1999; 26(5): 365-7 40. Wu X, Liu YK. Effect of ganglioside GM3 and GD3 on the cytotoxicity of mouse macrophage. Tumor 1998; 18(5):334-33641. He B, Liu YK, Tang ZY, Wu X, Zhang Y. Clinical significance of expressions of osteopontin in hepatocellular carcinoma. Chin J Hepatol, 1998;6(4):208-10 542. Zhu J, Zhou JP, Liu YK, Wu X, Zhang JH. The expression of protooncogene c-jun, c-fos in the mouse endometrium during the periimplantation and postimplantation period. Journal of reproductive medicine. 1998; 7(4): 231-443. Zhu J, Zhou JP, Liu YK, Wu X, Zhang JH. The expression of protooncogene c-jun, c-fos in the human early pregnant chorion and decidua. Chin J Obstet Gynecol, 1998; 33(4): 240-1 44. Wu JP, Zhang JH, Liu YK, Wu X, Zhou JP. The Expression of Fibronetin in Endometrium before and after Embryo Implantation. Reproduction and Contraception, 1998; 18 (4) : 205-9 Book ChaptersLunxiu Qin, Xin Wu et al. Molecular Diagnosis and prediction of Cancer. Shanghai Scientific & Technological Education Press, China, 2004JOURNAL REVIEWEREditorial Board. Gastroenterology Research. 2010Editorial Board. The Internet Journal of Oncology. 2010Invited Reviewer. Methods. 2010Reviewer of The American Journal of PathologyCell Biology InternationalJournal of Experimental & Clinical Cancer Research Cancer ResearchModern PathologyDiagnostic Molecular PathologyJournal of Hematology & OncologyJournal of CarcinogenesisJournal of RNAi and Gene SilencingGene Regulation and Systems BiologyRadiation OncologyOncotargetTECHNICAL SKILLSMolecular biology: PCR-Select cDNA Subtraction, Yeast two-hybrid, RACE-PCR, Real time PCR, MS- PCR, RT-PCR, Mutagenesis, Southern blotting, cDNA microarray, cDNA library construction, Northern blotting, Adeno-associated Virus (AAV), Adenovirus (AV), Lentivirus and Retrovirus construction, virus titration and infection, siRNAs and miRNA vector construction and transfection, DNA methylation, Recombinant protein expression in E. coli, Luciferase assay, In situ hybridization Protein biochemistry & Immunology: Humanized monoclonal antibody production and protein purification using AKTA Explorer, Avant and Pure, Agilent 1260 Infinity HPLC as well as Waters ACQUITY Premier UPLC System, immunoprecipitation (IP), Western blotting, ELISA, Immunohistochemistry (include immunofluorescence)Cell biology: Cell cycle, apoptosis analysis and cell sorting by FACS, multichannel flow cytometry for HSCT and hematology malignant diagnosis, Confocal microscopy, Fluorescence microscopy, cell migration assay, Colony forming assay, MTT,Animal experiment: Zinc finger nuclease and CRISPR technology to establish knock-in, knock-out mice and cell line, genotyping, xenograft, histology techniques. Glycolipid: Ganglioside extraction, Thin layer chromatography (TLC), Dialysis, Affinity chromatographyBioinformatics: TCGA database, RNA-seq and microarray data analysis using R and Bioconductor Computer skills: Office: Excel, Powerpoint, Word6Database: SQLiteStatistic: R, SAS, MATLAB, GraphPad PrismProgramming language: C++, C, Java, assembly language Web development: HTML, CSS,Others: PhotoshopReferenceYanyun Wu, M.D.Chief Medical OfficerBloodworks Northwest921 Terry Ave, Seattle WAMain line: PHONE NUMBER AVAILABLEDirect line: PHONE NUMBER AVAILABLEEmail: EMAIL AVAILABLEGregory A. Otterson, M.D., F.A.C.P.Professor, Internal MedicineB450 Starling Loving Hall320 West 10th AvenueColumbus OH 43210Tel: PHONE NUMBER AVAILABLEFax: PHONE NUMBER AVAILABLEEmail: EMAIL AVAILABLETakeshi Kurita. Ph DAssociate Professor, Cancer Biology & GeneticsJames Cancer Hospital and Solove Research Institute 812 Graves Hall460 W. 12th AveColumbus, Ohio 43210Phone: PHONE NUMBER AVAILABLEEmail: EMAIL AVAILABLE