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Candidate's Name
Street Address   Ph: PHONE NUMBER AVAILABLE/PHONE NUMBER AVAILABLE  EMAIL AVAILABLEWork Authorization Type  Green CardOBJECTIVE  Contribute and excel in life and contribute my skills to humanity.WORK EXPERIENCE
DEPARTMENT OF SURGERY, UNIVERSITY OF MINNEASOTA Minneapolis, MN, USAResearch Associate 01/11/2019 to dateCancer cell line culture, extraction and purification of exosomes, and molecular gene expression studies of colon cancer.DEPARTMENT OF VETERINARY MEDICINE, UNIVERSITY OF MINNEASOTA Minneapolis, MN, USAResearch Assistant (Veterinary Disease Diagnostic Lab) June-2014 to Oct-2014DNA and RNA extraction, Nucleic acid purification, PCR amplification and electrophoresis of the samples for diagnosis of viral disease in veterinary disease diagnosis lab.BAIF DEVELOPMENT RESEARCH FOUNDATION Pune, IndiaVeterinary Officer April-2013 to Aug-2013Oocytes In-vitro fertilization and maturation, embryo freezing, embryo transfer to surrogate mothers.Semen freezing and quality control analysis. Data management and clinical researchGUPTE HOSPITALS AND CENTER FOR RESEARCH IN REPRODUCTION Pune, IndiaClinical Embryologist Dec-2007 to June-2008Semen Analysis/Freezing, IUI, AID, IVF & IVM reproduction techniquesData and clinical research management  CRF filing, Maintaining GLP.RUBY HALL CLINIC AND RESEARCH CENTER Pune, IndiaClinical Embryologist Sep-2006 to June-2007Semen Analysis/Freezing, IUI, AID, IVF & IVM reproduction techniquesData and clinical research management  CRF filing, Maintaining GLP.PHOENIX POULTRY Jabalpur, IndiaVisiting Consultant Mar-2004 to May-2006Serological techniques, post-mortem examination procedures, histopathology sample preparation, virus & bacterial culture isolation and propagation. Implement vaccine trials. Scientific publications.Field investigations and poultry health management.EDUCATION
Biotechnology Center, JNKVV UNIVERSITY Jabalpur, IndiaMS in Animal Biotechnology(equivalent to Masters in Animal Biotechnology)Cumulative CGPA 8.4, Awarded merit scholarshipResearch/Thesis: Conservation of skin fibroblast cell lines and oocyte vitrification with its parthenogenetic development  A step towards cloning Barbari Goat.The objective of research was (1) to create and cryopreserve fibroblast cell lines of Barbari goat and compare its growth pattern of controlled cell line with epidermal growth factor treatment; (2) To compare DNA banding pattern of cell lines of different passage using RAPD markers (3) To cryopreserve immature oocyte by solid surface vitrification technique and test their developmental potential by in vitro maturation; (4) To test developmental potential of matured oocyte by parthenogenetic activation.Veterinary College Jabalpur, IndiaBachelors in Veterinary Science and Animal Husbandry(equivalent to Doctor of Veterinary Medicine DVM in USA)Cumulative CGPA 7.6, Awarded merit scholarshipUniversity of Pune Pune, IndiaCRCDM (Post graduate certificate course of Clinical Research & Clinical Data Management) with 74% from Pune University in the year 2007.Research/Thesis Stem Cells in Treatment of Different Clinical Conditions Special Reference to Bone Marrow Transplantation (BMT) or Hematopoietic Stem Cell Transplantation (HSCT).Biotechnology Center, JNKVV UNIVERSITY Jabalpur, IndiaCertificate training on 'Molecular Diagnosis of Viral Disease'.Virus isolation, virus cultivation, DNA and RNA isolation, cDNA preparation, RT, PCR, Transformation, gene-cloning and plasmid extraction. ELISA, DOT Blot, SDS-PAGE, western blotting, protein purification and estimation, automated DNA sequencing, mammalian cell culture etc.PUBLICATIONS
Vitrification of immature Goat oocytes by solid surface vitrification technique. Indian Journal AS. Vol77(7)-38-40.Vitrification of immature oocytes of Murrah Buffalo by SSV Technique. Buffalo bulletin Vol25-No-3(Sep-2006).Parthenogenetic Development of Barbari Goat oocyte by chemical activation. National Symposium, Karnal-India.Create and Cryopreserve the Skin Fibroblast Cell Lines of Murrah Buffalo. National Symposium, Karnal-India.Vitrification of immature oocytes of Barbari Goat by SSV Technique. National Symposium, Karnal-India.