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Click here or scroll down to respond to this candidate Candidate's Name .V
Phone No: PHONE NUMBER AVAILABLE
E-MAIL ID: EMAIL AVAILABLE
OBJECTIVE: Seeking a suitable and challenging job to put my skills and abilities for
better contribution of company that offers professional growth while being resourceful,
innovative and flexible.
EDUCATIONAL QUALIFICATION:
SL COURSE YEAR OF SCHOOL/ CLASS/ REMARK
NO. PASSING COLLEGE GRADE (%)
1 B.E 2012 Sir.MVIT 71.79 FCD
Vijaya PU First class
College,
2. PUC 2008 (60.33)
Bangalore
Florence High First class Distinction in
school, Maths, Science,
3 SSLC 2006 (80.80)
Social
Bangalore
TECHINICAL PROFICIENCY:
MOLECULAR BIOLOGY AND GENETIC ENGINEERING
Isolation of plasmid DNA from E.Coli cells.
Isolation of genomic DNA from Bacteria , Plants
Isolation of total RNA from plants.
Restriction digestion of plasmid and Agarose gel electrophoresis
Restriction digestion of Genomic DNA, Agarose gel electrophoresis and isolation of
Gene of interest.
Preparation of competent cells and Transformation.
Screening of recombinant clones.
UPSTREAM PROCESSING
Isolation of industrial important bacteria, yeast and fungi from SOIL, WATER and AIR.
Microbial products formation :
o Production of Alcohol from fermentable sugars using Yeast.
o Production of Citric acid from Aspergilus Niger.
o Production of Vinegar from Alcohol.
o Production of Lactic acid using Lactobacillus bacteria.
Cultivation of algae (Spirulina).
Production of enzyme (Amylase) from fungal source.
Hairy root culture for production of secondary metabolites.
Invitro induction of Callus & multiple shoot from explants.
Anther culture for the production of haploid plants.
Protoplast isolation using plant material.
Establishment of suspension callus cultures (Horticulture/medicinal plant).
Animal cell culture by trypsinization.
Primary culture technique from chick embryo fibroblast.
MTT cell proliferation (cell viability assay) using human cell lines.
DOWNSTREAM PROCESSING AND PRODUCT RECOVERY
Cell disruption techniques for Proteins and Intracellular enzymes by
o Mechanical method.
o Chemical method.
o Enzymatic method.
Solid-liquid separation methods:
o Sedimentation by flocculating agents.
o Filtration.
o Centrifugation.
o Membrane Filtration (fruit juices).
Concentration of material
o Drying vacuum tray drier, Atmospheric Tray drier, Spray drier.
o Evaporation.
o Lyophilizer.
Product Enrichment Operations
o Extraction [liquid liquid extraction & Aqueous two phase extraction].
Separation and purification of products by
o Centrifugation.
o Chromatography TLC (protein separation), Column Chromatography, GC, HPLC.
o Gel Electrophoresis PAGE & SDS PAGE.
SOFTWARE AND PROFESSIONAL SKILLS:
Pharmacoinformatics: RASMOL, KEGG, CLUSATAL, BLAST
ACADEMIC PROJECT DETAILS:
Done a project on DNA fingerprinting in medicinal crop Asparagus plant using
RAPD markers at DNA Laboratory, Biocentre, Hulimavu, INDIA.
Identification and molecular gene cloning of fibrinolytic enzymes from BACILLUS
SUBLITLIS to E.Coli at plant biotechnology, GKVK, INDIA.
EXTRACURRICULUR ACTIVITIES AND ACHIEVEMENTS:
Conferences Attended:
National Conference on Medical Biotechnology and Clinical Research, JN Tata
Auditorium, IISC Bangalore.
CBDG Session lecture and software demonstration, jointly organized by SIR MVIT
and Apsara Innovations Pvt Ltd
National Level one day Nanotechnology Sensitization programme for young, at
Seshadripuram first Grade College.
Won Prizes in Athletic and Dance competition at school level
Was a part of the Organizing committee of National Conference on Molecular
Medicine and Nano Biotechnology held at Nimhans Convention Center, Bangalore.
ADDRESS FOR COMMUNICATION:
Candidate's Name .V
22, CEDAR STREET,
HICKSVILLE, NY-11801
DATE:
(Candidate's Name . V )
PLACE: Hicksville, NY
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