Execute, develop, and optimize high throughput cell-based in vitro screening assay targeting deoxyribonucleic acid (DNA) endpoint sequencing. Characterize and quantify gene editing events for therapeutic drug discovery and its associated safety evaluation. Focus on high-throughput cell-based in vitro assays that utilize a variety of tissue- and cell-specific transfection-based methods that apply next generation sequencing (NGS) technologies, enabling massively parallel DNA sequencing to achieve the accuracy and sensitivity necessary to evaluate on-target editing events. Execute scientific experiments to produce data. Validate, optimize, automate, and scale in vitro screening methods for the detection of CRISPR/Cas9 genome editing. Optimize, scale, and validate screening sequencing methods as well as to validate liquid-handling platforms and software in multi-step biological assays. Design, implement and troubleshoot experiments, both independently and in collaboration with colleagues to reach Intellia's desired goals. Operate sophisticated, expensive, and sensitive liquid handling robotics including the Integra ViaFlow and the Hamilton MicroLab Star and massively parallel DNA sequencing machines requiring microfluidics. Provide support to guide RNA and messenger RNA chemistry platform as well as the lipid nanoparticle formulation platform by implementing in vitro assays to demonstrate potential therapeutic impact of various chemical and compositional modifications. Develop methods to support screening for insertion, excision, and alternative nuclease gene editing programs. Any remaining time is spent on data analysis, interpretation and in meetings with key stakeholders to communicate data and further Intellia’s understanding and development of a broad class of CRISPR/Cas9 gene therapies. Conduct standard cell culture support. Analyze aligned sequencing data and synthesize conclusions to help with the identification of future directions. Position is fixed based in Cambridge, MA office; However, telecommuting from a home office location is allowed.
40 hours/week, 9:00am-5:00pm.
Requires a Master’s degree (or foreign equivalent) in Biology, Biotechnology, Biological Sciences, or a directly related field plus two (2) years of laboratory experience in a cellular biology role involving the manipulation of cell-based systems.

Must have one (1) year of experience in the following (experience may be gained concurrently):
- Experience in sterile technique and tissue culture of immortalized and primary cell lines
- Experience with high-throughput liquid handling automation, including Hamilton and Integra
- Experience with nucleic acid purification and manipulation to create NGS libraries and preparation workflows, including polymerase chain reaction (PCR)
- Experience with DNA or RNA quantification through quantitative real-time
- Experience with immortalized and primary cell line culture and genetic perturbation through nucleic acid transfection or electroporation

Will accept a Bachelor’s degree (or foreign equivalent) plus five (5) years laboratory experience in a cellular biology role involving the manipulation of cell-based systems role in lieu of a Master’s degree plus two (2) years of experience.
Please send us your resume via email at candidates (at) placementservicesusa (dot) com with reference #2703515. Do not copy and paste your resume in the body email. Please attach it as a Word document. Thank you.